Fig. 1

Microorganism composition of the kefir. a Distribution pie chart of microorganisms in the kefir, obtained through total DNA shotgun sequencing based on a BLAST comparison in the One Codex data platform for applied microbial genomics. b Imagestream® flow cytometry analysis depicting microorganisms size/shape distribution. The dot plot of autofluorescence vs. bright field detail intensities of 19380 events analyzed. The brightfield cell images were used to draw an initial dot plot to identify cells of interest and exclude debris. The populations corresponding to initial hand-picked galleries of images are automatically gated on dot plot and each gate is indicated with a different color. Representative microscopic images of the cell subpopulations presented in the scatterplot: (i) bacterial cells; (ii) fungal cells; (iii–iv) fungal/bacterial cell aggregates. Scale bars correspond to 5 μm