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Fig. 1 | Microbiome

Fig. 1

From: Holo-omics for deciphering plant-microbiome interactions

Fig. 1

Considerations for design, analysis, and validation of holo-omic experiments. At left, design related considerations include intentional use of longitudinal designs, appropriate selection of sample types, and evaluation of optimal data types for the scientific questions addressed in the study. In the middle, analysis related challenges include selecting the appropriate range of biological and technical expertise, as well as the selection of appropriate analytical framework and tools for direct integration of diverse data types. At right, recommendations of techniques for downstream hypothesis testing and validation include use of direct and evolution-driven modifications of host genetic space, direct manipulation of microbial genetics, and bottom-up construction of reduced complexity synthetic communities

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