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Table 2 Table of exported functions and their descriptions available through the animalcules R package

From: animalcules: interactive microbiome analytics and visualization in R

Data and interface

 run_animalcules()

Initiates a local instance of the animalcules Shiny application

Data summary and manipulation

 filter_summary_bar_density()

Visualize sample/microbe data with a bar plot (categorical) or density plot (continuous)

 filter_summary_pie_box()

Visualize sample/microbe data with a pie chart (categorical) or box plot (continuous)

 filter_categorize()

Convert continuous variables into a various number of factors

 counts_to_logcpm()

Covert counts table to a log counts per million table

 counts_to_relabu()

Covert counts table to a relative abundances table

 upsample_counts()

Up-sample counts table to a higher taxon level

 find_taxonomy()

Find taxonomy for unlimited ids

 find_taxon_mat()

Find taxonomy information matrix for unlimited ids

 mae_pick_samples()

Isolate or discard samples from a multi-assay experiment object

 mae_pick_organisms()

Isolate or discard microbes from a multi-assay experiment object

Sample level visualization

 relabu_barplot()

Generate stacked bar plots of sample and group level microbe relative abundances

 relabu_boxplot()

Generate box plots comparing organism prevalence across groups of samples

 relabu_heatmap()

Generate a sample by microbe heatmap of counts

 dimred_pca()

Return a 2D/3D scatter plot for dimensionality reduction through PCA

 dimred_pcoa()

Return a 2D/3D scatter plot for dimensionality reduction through PCoA

 dimred_umap()

Return a 2D/3D scatter plot for dimensionality reduction through UMAP

 dimred_tsne()

Return a 2D/3D scatter plot for dimensionality reduction through t-SNE

Alpha and beta diversity

 diversities()

Return alpha diversity

 do_alpha_div_test()

Compute various statistical tests for alpha diversity

 alpha_div_boxplot()

Generate box plots comparing alpha diversity across groups of samples

 diversity_beta_test()

Compute various statistical tests for beta diversity

 diversity_beta_boxplot()

Generate box plots comparing beta diversity across groups of samples

 diversity_beta_heatmap()

Generate a heatmap comparing beta diversity across groups of samples

Differential abundance analysis

 differential_abundance()

Performs differential abundance analysis across groups of samples

Biomarker discovery

 find_biomarker()

Identifies microbes as potential biomarkers for groups of samples