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Fig. 4 | Microbiome

Fig. 4

From: Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individuals

Fig. 4

Species composition after contaminant and off-target removal. a Final species composition (absolute abundances) after removal of contaminants and off-target amplicons. Abundances were normalized with final 16S rRNA gene copy number and are characterized by an extremely low biomass. Highest biomass was observed in the samples where the DNA was extracted under the PK2 protocol. Mg, mouse; PD, Parkinson; HC, healthy control; SC, sterile brain from healthy living volunteers. b The three most sign. enriched zOTUs in study groups after automatic off-target amplicon removal. In-depth checks with manual Blast revealed that these most abundant zOTUs mapped to the human or the mouse genome or were likely contaminant taxa (see text)

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Microbiome

ISSN: 2049-2618

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