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Fig. 2 | Microbiome

Fig. 2

From: Much ado about nothing? Off-target amplification can lead to false-positive bacterial brain microbiome detection in healthy and Parkinson’s disease individuals

Fig. 2

Computational contamination identification. a Different approaches used to detect exogenous contamination; iNC, decontam “isNotcontaminant”; iC1, decontam “isContaminant” based on prevalence in negative controls; iC2, decontam “isContaminant” based on frequency in negative controls; iC3, decontam “isContaminant” based on combined frequency and prevalence in negative controls; Mouse, off-target zOTUs assigned to the mouse genome; Human, off-target zOTUs assigned to the human genome; Mock, contaminant taxa found in mock community samples; Contaminants, selection of known contaminant taxa in DNA extraction reagents (Suppl. Table 1); Abundance, taxa present in negative controls with relative abundances of ≥ 0.01. Set size represents absolute number of contaminants detected using each method. Intersection size represents the number of uniquely detected zOTUs. All detected contaminants were removed from the further analysis. b Relative and absolute abundances of contaminants, off-target amplicons, and remaining zOTUs in all study samples

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