Fig. 3

a, b Succession of resistomes with time during composting of dairy (closed shapes) and beef (open shapes) manure as illustrated by nonmetric multidimensional scaling of Bray Curtis distances of ARG annotations. Antibiotic versus no-antibiotic conditions are not labeled because there were no significant differences for either manure type or time point. Significant factors included a small-scale: duration of composting (R = 0.4, p = 0.001) and compost type (static or turned) (R = 0.1, p = 0.008) and b externally heated: duration of composting (R = 0.7, p = 0.001)