Table 1 DNA yield, trimmed reads and assembled contigs from the optimized phage extraction routes
Sample | DNA (ng/μl) | Trimmed Reads (Mill) | % of Reads surviving | Contigs (> 5 kb) | Average kb | Nucleotides assembled (Mb) |
|---|---|---|---|---|---|---|
0.22 μm TFF | 2.7 | 104 | 94.2 | 17,698 | 11.22 | 196.5 |
0.22 μm PEG | 1.70 | 60 | 92.4 | 7,583 | 11.15 | 84.5 |
0.45 μm TFF | 56.6 | 83 | 91.0 | 12,453 | 11.58 | 164.0 |
0.45 μm PEG | 2.44 | 64 | 92.9 | 10,493 | 13.17 | 121.4 |