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Fig. 3 | Microbiome

Fig. 3

From: Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil

Fig. 3

Effect of filtration pore sizes on bacteriophage recovery and bacterial contamination. Recovery of spiked bacteriophages (a) and respective bacterial DNA contamination (b) with a 0.22 μm or 0.45 μm PES filtration. Spiked bacteriophages were detected after bacteriophage elution and filtration of the soil suspension using plaque assay. Bacterial DNA contamination was assessed using Taqman qPCR amplifying the 16S rRNA gene. Boxplot and whiskers (min to max) with ns equals p = 0.7782 (n = 9), **** equals p < 0.0001 (n = 6).

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Microbiome

ISSN: 2049-2618

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