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Fig. 2 | Microbiome

Fig. 2

From: Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil

Fig. 2

Bacteriophage elution buffers for soil samples. Bacteriophage elution from soil samples was assessed for different elution buffers. The ability to elute phages from soil samples was tested for elution buffers previously published in literature or novel constituted elution buffer. Soil samples were spiked with an artificial viral community (A511, 2638AΔLCR, T7 at 106 PFU/g soil) and bacteriophage yield was assessed using plaque assay. (a) SM buffer [11, 23]; 10% beef extract buffer [5, 24]; PBS supplemented with beef extract [18]; AKC [1]. (b) Elution amelioration measured by plaque assay (mean ± SD) when using optimized PPBS instead of literature proposed elution buffer.

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