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Table 2 Fixatives and fixation times used on pure cultures

From: A pipeline for targeted metagenomics of environmental bacteria

 FixativeConcentrationTemperatureTime
1Formaldehyde4%Room temperature1 h
2Formaldehyde1%Room temperature1 h
3Formaldehyde0.25%Ice10 min
4Formaldehyde0.1%Ice10 min
5Ethanol70%Room temperature1 h
6Lugol’s solution2%Room temperature1 h
7Lugol’s and sodium thiosulfate2% and 0.07 MRoom temperature1 h
8Glyoxal4%Room temperature1 h
9Glyoxal and ethanol4% and 5%Room temperature1 h
10Control