Table 2 Fixatives and fixation times used on pure cultures

1

Formaldehyde

4%

Room temperature

1 h

2

Formaldehyde

1%

Room temperature

1 h

3

Formaldehyde

0.25%

Ice

10 min

4

Formaldehyde

0.1%

Ice

10 min

5

Ethanol

70%

Room temperature

1 h

6

Lugol’s solution

2%

Room temperature

1 h

7

Lugol’s and sodium thiosulfate

2% and 0.07 M

Room temperature

1 h

8

Glyoxal

4%

Room temperature

1 h

9

Glyoxal and ethanol

4% and 5%

Room temperature

1 h

10

Control

–

–

–