Fig. 4
From: A pipeline for targeted metagenomics of environmental bacteria
Sort criteria (gates) for flow cytometric sorting of ethanol fixed samples. A top: CF319a probe (Bacteroidetes); A bottom: Non338 probe (not binding, negative control); B top: Vis6-814 and Vis6-871 probe mix (Vis6-clade), B bottom: non338 probe. The blue fluorescence (355 nm laser, 460/50 nm detector) shows all bacteria stained with DAPI, the green fluorescence (488 nm laser, 530/40 nm detector) reveals probe-conferred signals. The percentage indicates the number of total events (500,000) detected within the sort gate. Note that in top panels a clearly higher percentage of FISH-positive signals for Bacteroidetes and Vis6 are visible above the background compared to bottom panels showing the control with the Non338 probe