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Fig. 5 | Microbiome

Fig. 5

From: Accurate identification and quantification of commensal microbiota bound by host immunoglobulins

Fig. 5

IgA-Seq in a mouse model of colitis with a defined 12-member microbiota. MM12 mice were administered weekly injections of anti-IL10R (day 0, 7). Controls received anti-1L10R alone (aIL10R, n = 4), and the colitis group were additionally infected with H. hepaticus at the start of the experiment (Hh+aIL10R, n = 4). a Experimental design and representative colon histology with haematoxylin and eosin staining. b Mouse lipocalin-2 ELISA as a measure of colonic inflammation. Lines at means and SEM. **p < 0.01 unpaired Mann-Whitney test, ns non-significant. SS represents steady state mice with no aIL10R exposure. c Representative flow cytometry plots of the percentage of colonic MM12 bacteria coated by IgA. d Percentage of bacteria coated by host IgA and subjected to IgA-Seq. P values represent Mann-Whitney tests. e Relative abundance plots showing the MM12 species in each sample. Each row represents an individual animal. f Non-metric multi-dimensional scaling of pre-sort, IgA+ and IgA fractions from Bray-Curtis distances. g Left: means and 95% confidence intervals of IgA binding scores significantly different between groups. Significance determined by complete permutation of group labels where, exact permuted p < 0.1). Right: Mean difference of scores between groups (colitic -control). Mean is normalised by its standard deviation for comparability between scoring methods (using the strictly standardised mean difference SSMD). h Left: boxplots of A. muciniphila relative abundance. Centre: boxplots of IgA+ fraction size. Right: boxplots of adjustment for the difference in fraction size (abundance in the IgA+ fraction size multiplied by A. muciniphila abundance). In the boxplots in (d) and (h), boxes represent the median and IQR and the whiskers the largest and smallest values within one and half IQR of the upper and lower IQR limits

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