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Fig. 2 | Microbiome

Fig. 2

From: Accurate identification and quantification of commensal microbiota bound by host immunoglobulins

Fig. 2

Testing IgA-Seq specificity in a complex faecal microbial community. Culture-grown Bt VPI-5482 was stained with mouse anti-Bt VPI-4582 IgA (clone 255.4) and rat anti-mouse IgA (PE, clone mA-6E1), then spiked into an unstained faecal suspension prepared from SPF C57BL/6J mice with a complex microbiota. a Experimental design and sort setup, gated on SYBR+ cells. b Sorts repeated on the same Bt-stool suspension to generate technical triplicates. Left: percentage of IgA+ bacteria in the pre-sorted sample, IgA+ and IgA fractions. Right: purity check on the collected fractions. Lines represent means with SEM. c Taxa abundances based on 16S rRNA gene sequencing of a complex stool microbiome (triplicate sequencing replications stool 1–3), triplicate sorted IgA+ fractions (sort 1–3) and Bt genomic DNA (Bt gDNA). Top 10 genera are shown. d Relative abundance of Bt VPI-5482 (ASV399) in sorted samples

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