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Fig. 2 | Microbiome

Fig. 2

From: Gut microbiota-derived propionate mediates the neuroprotective effect of osteocalcin in a mouse model of Parkinson’s disease

Fig. 2

Antibiotic pretreatment (Abx) blocked the OCN-induced improvements of motor impairments and dopaminergic neuronal loss in 6-OHDA-induced PD mice. a The experimental design for antibiotic treatment and 4 μg/kg OCN administration in 6-OHDA-induced PD mice. b (Upper panel) Bar plots of performance in the behavioral tests including the open field test, cylinder test, and rotarod test. n = 11-14 per group. (Lower panel) Representative traces in the open field test. Blue point: starting position; red point: ending position. c (Left panel) Representative immunostaining showing TH-positive neurons in the SN. (Right panel) The average number of TH-positive neurons in the ST. n = 3 per group, 3 sections per mouse. d (Left panel) Representative immunostaining (upper) and western blotting (lower) showing TH-positive fibers and TH protein levels in the striatum. (Right panel) The quantitation of TH-positive fibers (upper) and TH protein levels (lower) in the striatum. Immunostaining: n = 3 per group, 3 sections per mouse; western blotting: n = 3 per group. The data represent the mean ± SEM, p < 0.05 was set as the threshold for significance by one-way ANOVA followed by post hoc comparisons using Tukey’s test for multiple groups’ comparisons, *p < 0.05, **p < 0.01, *** p < 0.001, ****p < 0.0001

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