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Fig. 4 | Microbiome

Fig. 4

From: Subcellular view of host–microbiome nutrient exchange in sponges: insights into the ecological success of an early metazoan–microbe symbiosis

Fig. 4

Quantification of 13C and 15N incorporation by host cells and sponge-associated microbes of the HMA species P. angulospiculatus and LMA species H. caerulea after a pulse of isotopically labelled DOM (a–d) and POM (e–h) using NanoSIMS. Shown are mean 13C- (a–b, e–f) and 15N- (c–d, g–h) enrichment values (in Atom%) for three regions of interest (ROI) within the sponge tissue: choanocyte, mesohyl cell and sponge-associated microbe, at the end of the pulse (shaded grey area) and chase period. Box plots display data as quartiles (lower and upper hinges represent the 25th and 75th percentiles) for each ROI and black circles represent mean values. The horizontal grey lines show the natural variation in ROI from unlabelled control sponges (as mean ± SD). For details see Additional file 2: Table S1. Note the different y axis scale between carbon and nitrogen values

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Microbiome

ISSN: 2049-2618

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