Fig. 6
From: Discovery of novel community-relevant small proteins in a simplified human intestinal microbiome
Validation of sProtein identification with synthetic peptides. a The novel sProtein BT6 (57 aa) predicted by Prodigal and detected with the unique, fully tryptic peptide RSQLEHEVSVAQER is shown, as well as b the MS/MS spectrum which was assigned to the peptide spectrum match (PSM) by the search algorithm. c The unique peptide was synthesized and a MS/MS spectrum was generated. d Both spectra were compared using NIST’s MS search software, which resulted in a match factor of 814 and reverse match factor of 924, well above the cut-off values, thereby confirming expression of the novel sProtein BT6