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Fig. 3 | Microbiome

Fig. 3

From: Discovery of novel community-relevant small proteins in a simplified human intestinal microbiome

Fig. 3

Computational filtering strategy to identify potential novel sProteins. a The proteomics data was searched against the multi-species iPtgxDB using SequestHT and MS-GF+ separately. A stringent protein FDR < 0.01 was applied resulting in 6576 total proteins. In total, 904 of these were sProteins of which 253 were not contained in the NCBI RefSeq annotation. An additional prediction resource quality filter (Prodigal und ChemGenome predictions ≥ 3 PSMs; in silico ORF prediction ≥ 4 PSMs) resulted in 31 novel sProteins. b Comparison of the number of novel sProteins identified using trypsin and Asp-N as proteolytic enzymes before LC-MS/MS

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