Fig. 5

STRING network analysis [67] of expression data from colon showed different GOS effects on the intestinal epithelium of old and young mice. a GOS induced expression of binding-related genes (GO:0005488) in young mice while inducing b small-molecule metabolic processes genes (GO:0044281) in old animals. Network nodes represent predicted proteins. Splice isoforms or post-translational modifications are collapsed so each node represents all the proteins produced by a single, protein-coding gene locus. The confidence cutoff for showing interaction links was 0.900 (highest). The lower panel in a shows the most represented GO categories within binding-related genes in our transcriptomics data. c A heatmap of expression data revealed that GOS act as a modulator of the immune system in old and young mice. The heatmap was generated using ClustVis [68]. Rows were centered; unit variance scaling was applied to rows. Rows were clustered using correlation distance and average linkage