Fig. 6
RNA-seq analysis of BtVPI-5482, MD33MG, and MD40HG cultured in YM. a Log2 expression ratios of each transcript of BtVPI-5482, MD33MG, and MD40HG. Length of heap map is representative of genome size (BtVPI-5482 = 6.26 Mb, MD33MG = 6.28 Mb, MD40HG = 6.16 Mb). Location of MAN-PULs and MD40-PUL55 (white star) shown. Yellow indicates an increase in transcript expression compared to the control, while blue is decreased expression. b TPM values of susC-like genes from MAN-PUL1/2/3 showing if values within each gene are significantly different between the strains; different letters represent statistically significant values (e.g., A vs B) for each separate gene histogram; asterisk indicates SPII predicted proteins. Log2 fold change compares gene expression of YM cultures normalized to mannose, N = 3, p ≤ 0.01; except BT_3853 and HMNG-PUL, gene expression is not significantly (p > 0.05) different between the two treatments. Significantly different (p < 0.05) TPM expression of the susC-like genes between BtVPI-5482 and BtBov strains; statistical comparison of other MAN-PUL genes between strains were not calculated