Fig. 5

Divergence of MAN-PUL SusC/D-like proteins. a Phylogenetic tree of all the SusC-like MAN-PUL protein sequences from BtVPI-5482 (grey), MD33_MG (orange), and MD40_HG (blue). Phylogenetic trees of b SusC-like and c SusD-like amino acid sequences encoded in MAN-PUL2 of each Bt^Bov strain. Bootstrap values above 70% are indicated at branch points. Scale represents number of amino acid substitutions per site. d Growth of BtVPI-5482 wild-type and MAN-PUL susC/D-like mutants on 0.5% YM-MM. e Enumeration (N = 30,000) and f epifluorescence microscopy images of the uptake of FLA-YM by BtVPI-5482 wild-type and mutant strains after 2 h incubation. Cells counterstained with DAPI (blue)