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Fig. 5 | Microbiome

Fig. 5

From: Quantifying fluorescent glycan uptake to elucidate strain-level variability in foraging behaviors of rumen bacteria

Fig. 5

Divergence of MAN-PUL SusC/D-like proteins. a Phylogenetic tree of all the SusC-like MAN-PUL protein sequences from BtVPI-5482 (grey), MD33MG (orange), and MD40HG (blue). Phylogenetic trees of b SusC-like and c SusD-like amino acid sequences encoded in MAN-PUL2 of each BtBov strain. Bootstrap values above 70% are indicated at branch points. Scale represents number of amino acid substitutions per site. d Growth of BtVPI-5482 wild-type and MAN-PUL susC/D-like mutants on 0.5% YM-MM. e Enumeration (N = 30,000) and f epifluorescence microscopy images of the uptake of FLA-YM by BtVPI-5482 wild-type and mutant strains after 2 h incubation. Cells counterstained with DAPI (blue)

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