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Fig. 2 | Microbiome

Fig. 2

From: Quantifying fluorescent glycan uptake to elucidate strain-level variability in foraging behaviors of rumen bacteria

Fig. 2

Characterization of BtBov isolates. a 16S rRNA gene sequence comparison of BtVPI-5482 and BtBov strains. Scale represents number of nucleotide changes across horizontal axis. b Growth profiles of BtVPI-5482, BtΔMAN-PUL1/2/3, MD33MG, and MD40HG grown on 0.5% YM-MM. c SR-SIM of B. theta strains 0-min and 60-min post-incubation with FLA-YM. Cells co-stained with Nile Red and DAPI. d Mean fluorescence of bacterial cells cultured in FLA-YM or YM-MM (control). **p value < 0.01; ***p value < 0.001; ns p value > 0.05. e Bars represent percentage of total cells showing an uptake of FLA-YM in cultures sampled over time. Green = increased FLA-YM uptake; grey = no uptake. Mean cell fluorescence represented by line graph; solid line = mean of cells with FLA-YM signal; dotted line = mean of cells showing no FLA-YM uptake

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