Next-generation diagnostics: virus capture facilitates a sensitive viral diagnosis for epizootic and zoonotic pathogens including SARS-CoV-2

Table 4 Proficiency test samples with information on viruses contained and found after VirBaits treatment and dataset size

Sample number	Sample matrix provided	Sample details	Total reads	Virus contained	Virus found VirBaits	Virus portion
X1	Animal sample with TRIzol	Spleen of ASFV-infected wild boar	644,441	ASFV	ASFV	0.01%
X2	Animal sample with TRIzol	Saliva sample of FMDV-infected cattle	23,870	FMDV	FMDV	72%
X3	Extracted RNA	RVFV vaccine strain MP-12 with Vero 76 cells	24,022	RVFV	RVFV	98%
X3	Extracted RNA	RVFV vaccine strain MP-12 with Vero 76 cells	24,022		NiV	0.5%
X4	Extracted RNA	Mix of RNA (10 μl each) of Ebolavirus, Marburgvirus, Nipah henipavirus	256,408	NiV	NiV	45%
				EOV	EOV	0.6%
				Marburgvirus	Marburgvirus	0.05%
X5	Cells with TRIzol	African green monkey kidney cells	269,701	None	None	0%

ISSN: 2049-2618