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Table 2 Metagenomic reads classified as chitinase-encoding gene sequences with MG-RAST

From: Functional metagenomics reveals differential chitin degradation and utilization features across free-living and host-associated marine microbiomes

Dataset Sample category # Reads MG-RASTa # Blastb (%)
Octocoral metagenome Eunicella gazella (healthy) 94 68 (72.3%)
Eunicella gazella (necrotized) 59 47 (79.7%)
Eunicella verrucosa 1458 795 (54.5%)
Leptogorgia sarmentosa 127 100 (79.4%)
Sediments 401 124 (30.9%)
Seawater 599 317 (52.9%)
Sponge metagenome Spongia officinalis 48 12 (25.0%)
Sediments 455 103 (22.6%)
Seawater 257 103 (40.1%)
  1. aNumber of unassembled metagenomic reads classified by MG-RAST as endo-chitinase (EC 3.1.2.14) coding sequences
  2. bProportion of chitinase reads classified by MG-RAST which were assigned to the domain Bacteria and returned chitinase-specific closest hits after blastx searches on NCBI. Figure 4 shows the taxonomic affiliation of these reads in detail