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Table 1 Chitin and chitin-derivative degradation capacities of marine sponge and octocoral-derived bacterial isolates

From: Functional metagenomics reveals differential chitin degradation and utilization features across free-living and host-associated marine microbiomes

Strain Identity Isolation sourcea Chitin degradationb Chitinase activity (units/mL)c chiA PCRd Genome sequencee
EndoS3 ExoS1 ExoS2
Mc110 Micrococcus sp. Sarcotragus spinosulus No
EL33 Aquimarina sp. Eunicella labiata ++ + ++ + FLRG00000000.1
EL43 Aquimarina sp. Eunicella labiata ++ + ++ + No
Aq349 Aquimarina sp. Sarcotragus spinosulus ++ + + + OMKB00000000.1
Aq78 Aquimarina sp. Sarcotragus spinosulus ++ + + + OMKA00000000.1
Aq135 Aquimarina sp. Ircinia variabilis + + + + OMKE00000000.1
Aq107 Aquimarina sp. Sarcotragus spinosulus + + OMKC00000000.1
EL57 Aliivibrio sp. Eunicella labiata + + + No
EL58 Aliivibrio sp. Eunicella labiata ++ + OMPC00000000.1
EL24 Enterovibrio sp. Eunicella labiata ++ + + + + No
EL37 Enterovibrio sp. Eunicella labiata ++ + + + + No
EL22 Vibrio sp. Eunicella labiata ++ ++ + + + No
EL36 Vibrio sp. Eunicella labiata + + + + + No
EL38 Vibrio sp. Eunicella labiata ++ ++ + + + No
EL41 Vibrio sp. Eunicella labiata ++ + + + No
EL49 Vibrio sp. Eunicella labiata ++ + + + + No
EL62 Vibrio sp. Eunicella labiata + + + + + No
EL67 Vibrio sp. Eunicella labiata + ++ + + + No
EL112 Vibrio sp. Eunicella labiata ++ + + + + No
Vb255 Vibrio sp. Sarcotragus spinosulus ++ + ++ + + Yese
Vb258 Vibrio sp. Sarcotragus spinosulus ++ + ++ + + Yesf
Vb341 Vibrio sp. Sarcotragus spinosulus + + + + - No
Vb339 Vibrio sp. Sarcotragus spinosulus + + + + + GCA_902751245.1
Vb278 Vibrio sp. Sarcotragus spinosulus ++ ++ + + + CVNE00000000.1
Cw315 Colwellia sp. Sarcotragus spinosulus + No
Pa284 Pseudoalteromonas sp. Sarcotragus spinosulus + + ++ No
EL12 Shewanella sp. Eunicella labiata + ++ + No
Sw66 Shewanella sp. Sarcotragus spinosulus + + + ++ No
Mb45 Microbulbifer sp. Sarcotragus spinosulus ++ + + + No
EL27 Pseudophaeobacter sp. Eunicella labiata OMPQ00000000.1
EL26 Roseovarius sp. Eunicella labiata OUMZ00000000.1
EL01 Ruegeria sp. Eunicella labiata OMPS00000000.1
Rg50 Ruegeria sp. Sarcotragus spinosulus + No
EL44 Sulfitobacter sp. Eunicella labiata OMPT00000000.1
EL53 uncl. Rhodobacteraceae Eunicella labiata + OMPR00000000.1
EL129 uncl. Rhodobacteraceae Eunicella labiata ONZJ00000000.1
Ph222 uncl. Rhodobacteraceae Ircinia variabilis No
EL143 Labrenzia alba Eunicella labiata + + OGUZ00000000.1
Pv125 Pseudovibrio sp. Sarcotragus spinosulus ++ No
EL199 Kiloniella sp. Eunicella labiata + + OMPU00000000.1
EL138 Sphingorhabdus sp. Eunicella labiata OGVD00000000.1
  1. aEunicella labiata: octocoral; Sarcotragus spinosulus and Ircinia variabilis: marine sponges
  2. bChitin degradation observed as halo formation on colloidal chitin-containing agar plates: ++ halo-radius ≥ 8 mm, + halo-radius ≥ 1 mm and < 8 mm
  3. cChitinase activity measured in Units/ml on bacterial culture supernatants (EndoS3 = endo-chitinase activity) or cell extracts (ExoS1 = exo-chitinase β-N-acetylglucosaminidase activity; ExoS2 = exo-chitinase chitobiosidase activity): ++ ≥ 1 U/mL, + ≥ 0.01 and < 1 U/mL
  4. dPCR-based amplification of the chiA gene fragments. eAccession numbers are provided for genome sequences available on NCBI
  5. e,fUnpublished draft genomes