Fig. 4
Proportion of representative genomes at the subspecies level with a given function among genomes detected in each sample within each floodplain. Box plots in each panel represent floodplain G (left), floodplain L (middle), and floodplain Z (right). a Most abundant functions: 1. Acetate formation, 2. Oxidation of CO and other small molecules, 3. Formate oxidation: CH2O2 to CO2 + H2, 4. Sulfide oxidation: H2S to S0. b Geochemical transformations in the Carbon cycle: 5. CO2 fixation pathways, 6. Anaerobic CO oxidation, 7. Methanol oxidation, 8. Formaldehyde oxidation pathways (see Table S4, Additional File 6). c Geochemical transformations in the sulfur cycle: 9. Sulfide oxidation (reverse dsr) from hydrogen sulfide: H2S to SO32-, 10. Sulfite oxidation to sulfate (or vice versa): SO32- to SO42-, 11. Thiosulfate oxidation without sulfur deposition: S2O32- to SO42-, 12. Thiosulfate oxidation with sulfur deposition: S2O32- to SO42- + S0. d The nitrogen cycle: 13. Nitrogen fixation: N2 to NH3, 14. Ammonia oxidation: NH3 to NH2OH, 15. Hydroxylamine oxidation (requires additional, undetermined enzyme): NH2OH to NO2-, 16. Nitrite oxidation: NO2- to NO3- (reversible), 17. Nitrate reduction (cytoplasmic): NO3- to NO2-, 18. Nitrate reduction (periplasmic): NO3- to NO2-, 19. Assimilatory nitrite reduction: NO2- to NH4, 20. Dissimilatory nitrite reduction: NO2- to NH4, 21. Assimilatory or dissimilatory nitrate reduction (ANRA or DNRA): 17 or 18 + 19 or 20, 22 & 23. Nitrite reduction (Denitrification): NO2- to NO, 24. Nitric oxide reduction: NO to N2O, 25. Nitrous oxide reduction: N2O to N2. e Hydrogen metabolism via hydrogenases: 26. FeFe hydrogenases group A (fermenting and bifurcating), 27. FeFe hydrogenases group C (H2 sensors), 28. NiFe hydrogenases group 1 (H2 oxidation), 29. NiFe hydrogenases group 2 (H2 oxidation), 30. NiFe hydrogenases group 3b (bidirectional), 31. NiFe hydrogenases group 3c (bidirectional), 32. NiFe hydrogenases group 3d (bidirectional). Paired, same color bars above boxplots indicate statistically significant differences between those two floodplains (two-way ANOVA)