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Table 3 mNGS and r(RT-)PCR results of 7 patients with clinically unrecognized viral sequences

From: Unmasking viral sequences by metagenomic next-generation sequencing in adult human blood samples during steroid-refractory/dependent graft-versus-host disease

 

Viral species detected with mNGS

Viral species confirmed with r(RT)-PCR assays

Patient’s code

Virus

Plasma, n (pos/total)

Time period (days)

Estimated viral load (mean log10 copies/ml plasma or CT values)

Other positive biological specimen

Ge02

HPyV7

14/14

279

4.75

BM, BAL

Ge05

Usutu virus

2/5

7

4.66

–

Ge07

Cutavirus

6/10

98

37.9

Skin

Ge09

Bufavirus

6/12

81

3.73

Stools, duodenum

Ge12

Novel human astrovirus MLB2

2/6

14

3.24

Intestine, colon, BM

Ge14

Rubella virus

3/5

39

37.9

–

Classic human astrovirus

5/11

25

5.56

–

HPyV6

11/11

74

3.76

–

Ge22

Rubella virus

2/8

3

36.7

–

  1. Pos/total corresponds to the number of positive samples on the total of screened samples. Time period corresponds to the time period during which samplings were found positive. CT values above 40 were considered negative. CT values are indicated in italics
  2. Abbreviations: BM bone marrow, BAL bronchoalveolar lavage, CT cycle threshold