Fig. 3
From: Modulating gut microbiota in a mouse model of Graves’ orbitopathy and its impact on induced disease
Mouse TSHR antibody evaluation and impact of gut microbiome modification on thyroid function and morphology. a Total TSHR antibodies (TRAbs) were measured by TSH binding inhibitory immunoglobulin activity to the human TSHR given as % inhibition of bTSH. b Thyroid-stimulating autoantibodies (TSAbs). Stimulating activity is given in cAMP (pmol/mL). Data are presented as mean ± standard error of the mean. Multiplicity adjusted P values are marked as follows ****P < 0.0001, ***P < 0.001, *P < 0.05 (two-way ANOVA). c Total T4 values (μg/dl) in βgal- and TSHR-immunized mice from each treatment group *P < 0.05. d H&E of thyroid slices for thyroid morphology evaluation (in normal, heterogeneous (hetero) or hyperthyroid (hyper)). e Representative thyroid images at × 20 magnification. Heterogeneous thyroids contained normal and hyperthyroid regions (indicated by arrows). f Weights of animals at the endpoint of the experiment. Data are presented as mean ± standard error of the mean. Multiplicity adjusted P value is marked as **P < 0.01 (two-way ANOVA)