Fig. 1
From: Modulating gut microbiota in a mouse model of Graves’ orbitopathy and its impact on induced disease
Early-life manipulation treatments modified the lower gastrointestinal microbiota with long-term effects in TSHR-immunized mice. a Rationale of early-life manipulation treatments combined with the hTSHR-immunization procedure, see STAR methods for complete description. b Alpha-diversity indices amongst treatments. Wilcoxon-Mann test BH corrected: ***P < 0.001; **P = 0.019; *P = 0.045. c Significantly differentially abundant genera between immunizations in each treatment. Welch’s t test, BH corrected: ***P < 0.001; **P = 0.011; *P = 0.04. d Random forest confusion matrix of the classification for treatments (all immunizations together). Diagonal boxes represent the number of samples correctly predicted. The RF model accounted for 73.08% accuracy (n mice/group endpoint: control = 20, hFMT = 19, Lab4 = 20, vancomycin = 19). e Top-10 important variables contributing to treatment classification according to the Mean Decrease Gini, related to e. f RandomForest confusion matrix of the classification for immunizations. The RF model accounted for 70.51% accuracy (n mice/immunization endpoint: TSHR = 43, βgal = 35). g Top-10 important variables contributing to immunization classification according to the Mean Decrease Gini, related to g