Fig. 2

Spearman rho correlation analysis of PC1 and PC2 with the most abundant or relevant OTUs. The color of each block in the heatmap indicates the strength of correlation between each OTU and PC1 or PC2, or with other OTUs. The 3 mock community samples were excluded. Likely, contaminants show strong correlations with PC1 and PC2, and with each other, and are indicated with the light blue vertical bar on the left. OTUs indicated with a single asterisk were identified by decontam as a contaminant and were removed after filtering. Double asterisk indicates that they were not identified by decontam but that they were removed after filtering, and triple asterisk indicates OTUs that were not identified by decontam and were also not removed after filtering. The light blue boxes within the heatmap indicate strong correlation with PC1 and/or PC2 (the batch effect) and/or that they were strongly correlated with one another and abundantly present in negative controls