Perinatal environment shapes microbiota colonization and infant growth: impact on host response and intestinal function

Table 1 Gene expression of cells in the apical and basal compartment in the triple co-culture system after 7 days of exposure

	HB		CS
	Relative expression	p value	Relative expression	p value
Apical compartment (Caco-2, LSTH-17 cells)
HP	1.139 (0.767–1.627)	0.495	1.097 (0.957–1.214)	0.106
CDH1	1.054 (0.462–1.675)	0.841	0.916 (0.736–1.237)	0.399
OCLN	1.163 (0.673–1.534)	0.531	1.321 (0.867–1.702)	0.083
IRAK4 (#)	0.94 (0.610–1.168)	0.837	0.425 (0.273–0.595)	0.010*↓
TLR2	1.492 (0.837–2.142)	0.181	1.302 (0.960–1.710)	0.061
TLR4	0.89 (0.722–1.074)	0.201	0.826 (0.482–1.276)	0.475
TOLLIP	0 (0.000–1.381)	0.509	1.231 (0.658–2.076)	0.474
Basal compartment (THP-1 cells)
TLR4	3.566 (1.103–13.629)	0.094	1.117 (0.235–7.013)	0.896
TLR3	1.346 (0.939–1.853)	0.378	3.546 (1.712–8.099)	0.094
TOLLIP	5.182 (3.667–9.351)	*< 0.001↑	7.186 (4.388–11.727)	0.010*↑
IL10	7.888 (3.496–31.769)	*< 0.001↑	1.737 (0.912–5.478)	0.061

Total RNA was extracted from cells treated with homebirth (HB) samples and C-section (CS) fecal supernatant. For each condition, n = 6 in triplicates, data was presented as fold change expression (95% C.I). Values show relative expression of each condition compared to control. P < 0.05 (*) and blond letters marked significant differences between treatment and control, up (↑) or down (↓) regulation was represented by the arrows. Symbol # represented genes expression that was different between HB and CS

ISSN: 2049-2618