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Fig. 6 | Microbiome

Fig. 6

From: Perinatal environment shapes microbiota colonization and infant growth: impact on host response and intestinal function

Fig. 6

Effect of fecal water long-term exposure (7 days) on the triple co-culture system. a, b Epithelial barrier function measured as trans-epithelial electric resistance (TEER) (a) and Lucifer yellow transport (LY) (b). c Mucus production by LSTH17 cells after the long-term exposure on the triple co-culture system measured by Bradford assay. d Interleukin (IL) 8 production by cells on the apical compartment (CacO-2 and LSTH17) measured by ELISA and expressed as increment respect to control condition. e, f Intestinal cells functional maturation degree measured as intestinal alkaline phosphatase activity (IAP) on apical compartment during the treatment (e) and at final time point (f). g, h Cytokine production in the basal compartment by THP-1 cells. IL-8 (g) and IL-6 (h) production after fecal supernatant long-term exposure expressed as increment respect to control condition. The treatments were fecal water from infants born by C-section (CS), vaginal delivery at hospital (VAG), and homebirth (HB). Non-normal data was presented as median and whiskers represented the 5–95 percentile while normal data was showed as mean and SD. Kruskal-Wallis/ANOVA and Dunn’s/Tukey’s post hoc (FDR adjustment) test was used to test the significance of the normal/non normal distributed variables between the groups. In the cytokine analysis, the symbol (*) represented variations between time within the same studied group according to the color. *p < 0.05, **p < 0.01, ***p < 0.001

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