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Table 4 Detection of acute clinical T. cruzi infections using UPDx with double digestion

From: Sensitive universal detection of blood parasites by selective pathogen-DNA enrichment and deep amplicon sequencing

Sample Tc readsa Total reads % Reads Result
Cultureb 4496 12,999 34.6% +
Acute1 64 8549 0.7% +
Acute2 0 4900 0.0%
Acute3 9189 10,731 85.6% +
Acute4 10,864 13,428 80.9% +
Acute5 9152 11,161 82.0% +
Reactivation1 0 7902 0.0%
Reactivation2 0 6372 0.0%
Reactivation3 0 9592 0.0%
Reactivation4 0 9822 0.0%
Reactivation5 0 8680 0.0%
  1. aThis is the sum of reads used to construct haplotypes that were identical to T. cruzi reference sequences AF288661.1 and/or CP015675.1
  2. bPositive control