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Table 1 Host, source, and original identification method of samples used in this study

From: Sensitive universal detection of blood parasites by selective pathogen-DNA enrichment and deep amplicon sequencing

Parasite Specimen number Sample type Host Species identification diagnostic method/s
Plasmodium falciparum Specimen 1 EDTA blood Homo sapiens Microscopy and PCR w/s [29]
Plasmodium vivax Specimen 2 EDTA blood Homo sapiens Microscopy and PCR w/s [29]
Plasmodium ovale Specimen 3 EDTA blood Homo sapiens Microscopy and PCR w/s [29]
Plasmodium malariae Specimen 4 EDTA blood Homo sapiens Microscopy and PCR w/s [29]
Plasmodium knowlesi Specimen 5 EDTA blood Macaca mulatta Microscopy
Babesia microti Specimen 6 EDTA blood Homo sapiens Microscopy and PCR w/s [30]
Babesia divergens Specimen 7 EDTA blood Meriones unguiculatus Microscopy and PCR w/s [30]
Babesia duncani Specimen 8 EDTA blood Meriones unguiculatus Microscopy and PCR w/s [30]
Babesia divergens-like variant MO1 Specimen 9 EDTA blood Homo sapiens Microscopy and PCR w/s [30]
Trypanosoma cruzi (infections) Specimens 10 and 11 EDTA blood Homo sapiens Real-time PCR (Qvarnstrom 2012)
Trypanosoma cruzi (culture) Specimen 12 RPMI culture in EDTA blood Homo sapiens Microscopy
Trypanosoma brucei Specimen 13 HMI-9 culture in EDTA blood Homo sapiens Microscopy
Leishmania infantum Specimen 14 RPMI culture in EDTA blood Homo sapiens Microscopy and PCR w/s [31]
Leishmania donovani Specimen 15 RPMI culture in EDTA blood Homo sapiens Microscopy and PCR w/s [31]
Brugia malayi Specimen 16 EDTA blood Felis catus Microscopy
Loa loa Specimen 17 EDTA blood Homo sapiens Real-time PCR [32]
Mansonella perstans Specimen 18 Blood spot on filter paper Homo sapiens Real-time PCR [33, 34]
NPF (no parasite found) N/A EDTA blood Homo sapiens Microscopy, PCR [29,30,31]
  1. w/s with Sanger sequencing of PCR product, CDC Centers for Disease Control and Prevention, FR3 Filariasis Research Reagent Resource Center, PDB Parasitic Diseases Branch, UGA University of Georgia, NIH National Institutes of Health