Fig. 3

UPDx with double digestion increases the number of parasite-derived reads recovered following TADS. Restriction enzyme digestion yields a substantial increase in the percentage of parasite-derived 18S rDNA reads from parasite-infected whole blood samples following PCR and TADS. The increase in parasite reads is most pronounced in samples undergoing both restriction Digest 1 and Digest 2, as evidenced by the percentage of parasite-derived reads recovered from blood samples containing apixomplexans (a), kinetoplastids (b), and microfilariae (c), (statistical analysis involved a 2-way ANOVA with Tukey’s multiple comparisons posttest, **** p < 0.0001, *** p < 0.001, ** p < 0.01, n = 3, mean ± SD)