Fig. 2

UPDx with double digestion significantly reduces the number of human-derived reads recovered following TADS. Restriction enzyme digestion yields a marked reduction in the percentage of human-derived 18S rDNA reads in parasite-infected blood samples following PCR and TADS. Human-derived read reduction is most pronounced in samples undergoing both restriction Digest 1 and Digest 2, as evidenced by the percentage of human-derived reads in most blood samples containing apixomplexan (a) and kinetoplastid (b) parasites. However, no visible reduction in human reads is observed in human blood displaying low parasitemia microfilarial infections (c, 2-way ANOVA with Tukey’s multiple comparisons posttest, **** p < 0.0001, *** p < 0.001, ** p < 0.01, n = 3, mean ± SD)