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Table 1 Genome features for 27 high quality and high contiguity MAGs recovered from the PNA ecosystem using the Iterative Hybrid Assembly (IHA) approach. Taxonomic assignments were identified using GTDB-Tk

From: High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

MAGs ID

Status

Genome size (Mbp)

N50 (Mbp)

No. of contigs

GC

C/C (%)

Features (CDS/rRNA/tRNA)

Taxonomy

H1_BAC1

 

3.379

3.379

1

0.460

98.5/0.3

3138/3/40

Bacteroidota (family Cyclobacteriaceae)

H1_PLA1

 

3.610

3.610

1

0.700

98.9/0.0

3039/3/49

Planctomycetota (order Phycisphaerales)

H1_PLA2

Circular

3.877

3.877

1

0.633

96.6/0.0

3140/3/51

Planctomycetota (class Phycisphaerae)

H1_AMX1

Circular

3.495

3.495

1

0.425

100.0/1.6

3099/3/47

Planctomycetota (genus Brocadia)

H1_AOB3

 

2.842

2.842

1

0.512

100.0/0.3

2674/3/43

Proteobacteria (genus Nitrosomonas)

H1_ARM1

Circular

2.897

2.897

1

0.609

94.9/0.9

2665/3/48

Armatimonadota (family Fimbriimonadaceae)

H1_PLA3

Circular

3.887

3.887

1

0.673

96.6/0.0

3032/3/53

Planctomycetota (class Phycisphaerae)

H1_PLA4

Circular

3.841

3.841

1

0.672

97.7/0.0

3229/3/54

Planctomycetota (order Phycisphaerales)

H1_AOB1

Circular

3.279

3.279

1

0.499

99.8/0.0

3122/3/38

Proteobacteria (genus Nitrosomonas)

H1_CFX2

 

4.791

4.742

4

0.531

93.6/3.6

4575/3/50

Chloroflexota (order Anaerolineales)

H1_AOB2

Circular

3.217

3.217

1

0.495

99.9/0.0

3074/3/39

Proteobacteria (genus Nitrosomonas)

H1_BAC2

 

2.842

2.842

1

0.495

97.0/1.1

2326/6/44

Bacteroidota (family Kapabacteriaceae)

H1_PLA5

 

5.011

3.408

4

0.610

97.7/1.1

4106/3/52

Planctomycetota (class Phycisphaerae)

H1_PLA6

 

5.026

3.822

2

0.650

97.7/1.7

4074/3/51

Planctomycetota (class Phycisphaerae)

H2_CFX3

 

4.684

1.286

5

0.566

100.0/2.7

3904/6/49

Chloroflexota (class Anaerolineae)

H2_MYX1

 

9.475

3.220

5

0.698

93.9/2.6

8465/3/79

Myxococcota (family Polyangiaceae)

H2_BAC3

Circular

4.213

4.213

1

0.441

98.9/0.0

3318/3/43

Bacteroidota (order Ignavibacteriales)

H2_PRO1

 

3.105

3.105

1

0.686

91.0/1.5

2810/3/51

Proteobacteria (class Gammaproteobacteria)

H3_ACI1

 

2.971

1.982

2

0.538

96.6/2.6

2688/3/47

Acidobacteriota (family Pyrinomonadaceae)

H3_CFX4

 

3.148

1.455

4

0.667

93.1/1.0

3207/3/48

Chloroflexota (class Dehalococcoidia)

H3_PLA7

 

3.869

0.987

5

0.653

94.3/3.4

3180/3/67

Planctomycetota

H5_SPI1

 

4.129

2.456

4

0.531

96.5/0.0

4087/3/40

Spirochaetota (order Leptospirales)

H5_BAC4

 

3.186

2.101

3

0.388

99.5/1.7

2670/3/42

Bacteroidota (class Bacteroidia)

H5_UNCL1

 

4.583

2.293

3

0.545

96.6/2.7

3627/3/49

BRC1 (species OLB16)

H5_CFX6

 

2.882

1.591

2

0.525

92.7/0.9

2714/3/48

Chloroflexota (order Anaerolineales)

H5_SPI2

 

3.423

2.007

4

0.346

100.0/0.0

3317/6/37

Spirochaetota (family Leptospiraceae)

H5_BAC6

 

3.824

2.408

4

0.348

99.4/0.0

3310/3/44

Bacteroidota (family Ignavibacteriaceae)

  1. Note: MAGs ID is short for the phylum-level taxonomy assigned using GTDB-Tk. The red asterisk means the genome was classified using the identified 16S rRNA, which had a significant improvement in classification. The column “C/C” stands for the completeness and contamination for each reconstructed MAG evaluated by using CheckM. The “Taxonomy” column contained the phylum level assignments, as well as the highest clade level annotations so far in the bracket