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Table 1 Genome features for 27 high quality and high contiguity MAGs recovered from the PNA ecosystem using the Iterative Hybrid Assembly (IHA) approach. Taxonomic assignments were identified using GTDB-Tk

From: High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

MAGs ID Status Genome size (Mbp) N50 (Mbp) No. of contigs GC C/C (%) Features (CDS/rRNA/tRNA) Taxonomy
H1_BAC1   3.379 3.379 1 0.460 98.5/0.3 3138/3/40 Bacteroidota (family Cyclobacteriaceae)
H1_PLA1   3.610 3.610 1 0.700 98.9/0.0 3039/3/49 Planctomycetota (order Phycisphaerales)
H1_PLA2 Circular 3.877 3.877 1 0.633 96.6/0.0 3140/3/51 Planctomycetota (class Phycisphaerae)
H1_AMX1 Circular 3.495 3.495 1 0.425 100.0/1.6 3099/3/47 Planctomycetota (genus Brocadia)
H1_AOB3   2.842 2.842 1 0.512 100.0/0.3 2674/3/43 Proteobacteria (genus Nitrosomonas)
H1_ARM1 Circular 2.897 2.897 1 0.609 94.9/0.9 2665/3/48 Armatimonadota (family Fimbriimonadaceae)
H1_PLA3 Circular 3.887 3.887 1 0.673 96.6/0.0 3032/3/53 Planctomycetota (class Phycisphaerae)
H1_PLA4 Circular 3.841 3.841 1 0.672 97.7/0.0 3229/3/54 Planctomycetota (order Phycisphaerales)
H1_AOB1 Circular 3.279 3.279 1 0.499 99.8/0.0 3122/3/38 Proteobacteria (genus Nitrosomonas)
H1_CFX2   4.791 4.742 4 0.531 93.6/3.6 4575/3/50 Chloroflexota (order Anaerolineales)
H1_AOB2 Circular 3.217 3.217 1 0.495 99.9/0.0 3074/3/39 Proteobacteria (genus Nitrosomonas)
H1_BAC2   2.842 2.842 1 0.495 97.0/1.1 2326/6/44 Bacteroidota (family Kapabacteriaceae)
H1_PLA5   5.011 3.408 4 0.610 97.7/1.1 4106/3/52 Planctomycetota (class Phycisphaerae)
H1_PLA6   5.026 3.822 2 0.650 97.7/1.7 4074/3/51 Planctomycetota (class Phycisphaerae)
H2_CFX3   4.684 1.286 5 0.566 100.0/2.7 3904/6/49 Chloroflexota (class Anaerolineae)
H2_MYX1   9.475 3.220 5 0.698 93.9/2.6 8465/3/79 Myxococcota (family Polyangiaceae)
H2_BAC3 Circular 4.213 4.213 1 0.441 98.9/0.0 3318/3/43 Bacteroidota (order Ignavibacteriales)
H2_PRO1   3.105 3.105 1 0.686 91.0/1.5 2810/3/51 Proteobacteria (class Gammaproteobacteria)
H3_ACI1   2.971 1.982 2 0.538 96.6/2.6 2688/3/47 Acidobacteriota (family Pyrinomonadaceae)
H3_CFX4   3.148 1.455 4 0.667 93.1/1.0 3207/3/48 Chloroflexota (class Dehalococcoidia)
H3_PLA7   3.869 0.987 5 0.653 94.3/3.4 3180/3/67 Planctomycetota
H5_SPI1   4.129 2.456 4 0.531 96.5/0.0 4087/3/40 Spirochaetota (order Leptospirales)
H5_BAC4   3.186 2.101 3 0.388 99.5/1.7 2670/3/42 Bacteroidota (class Bacteroidia)
H5_UNCL1   4.583 2.293 3 0.545 96.6/2.7 3627/3/49 BRC1 (species OLB16)
H5_CFX6   2.882 1.591 2 0.525 92.7/0.9 2714/3/48 Chloroflexota (order Anaerolineales)
H5_SPI2   3.423 2.007 4 0.346 100.0/0.0 3317/6/37 Spirochaetota (family Leptospiraceae)
H5_BAC6   3.824 2.408 4 0.348 99.4/0.0 3310/3/44 Bacteroidota (family Ignavibacteriaceae)
  1. Note: MAGs ID is short for the phylum-level taxonomy assigned using GTDB-Tk. The red asterisk means the genome was classified using the identified 16S rRNA, which had a significant improvement in classification. The column “C/C” stands for the completeness and contamination for each reconstructed MAG evaluated by using CheckM. The “Taxonomy” column contained the phylum level assignments, as well as the highest clade level annotations so far in the bracket
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