Fig. 2

Assembler performance evaluation using the PNA-with-Spiked-Mock dataset. a Genome recoveries by the four assemblers, megahit (n = 7), metaSPAdes (n = 7), OPERA-MS (n = 7), and Unicycler (n = 8). b Reconstructed genome purity and the aligned genome fraction against the reference genome. c Genome continuity (NGA50) for the four assemblers as a function of genome coverage in short-read dataset. d Misassembly event in the assembled genome (one circle, one genome), with the orange triangle and red dashed line indicating the average and median value of the assembled genome misassembly event, respectively. e Gene recovery ratio and percentages of genes assembled by the four assemblers