Fig 6.

Characterization of attraction points in the host-microbiota ecosystem model. a Stability of attraction points. For each of the quadrants 1A, 1B, 2A, and 2B from Fig. 5 the percentage of animals presenting a stable microbiota is indicated. Stable microbiota is defined as showing the same state (either basal state 1 or alternative state 2) at four consecutive sampling time-points (T31, T40, T63, T68; i.e., from just after the last DSS treatment onward). >, >>, and < signs are read from left to right and from top to bottom. *p = 0.003 (Fisher exact test). b Relative abundance of Akkermansia in different microbiota, host, and ecosystem states. Abundance is expressed as percentage of total number of sequence reads; median values for the rats in each state at T68 are represented. Statistically significant differences are indicated in red. Differences between ecosystem states (1A, 1B, 2A, 2B) were analyzed using a Kruskal-Wallis test with post hoc Dunn’s test and Holm correction for multiple comparisons; differences between microbiota states (1, 2) or host states (A, B) were analyzed using a Wilcoxon test with FDR adjustment. c Akkermansia, Bacteroides and Butyricimonas distributions in microbiota states 1 (m1) and 2 (m2). Combined data from T-7 to T68 (n = 463 samples for microbiota state 1, n = 99 samples for microbiota state 2 (cf Table 1)); each dot represents one intestinal microbiota sample. Abundance is expressed as number of sequence reads on a total of 38,000. Only genera for which the median abundances in the two microbiota states differ at least 1.2-fold with q < 0.05 (Wilcoxon test with FDR adjustment) are presented. d Spearman correlation (r) between Akkermansia, Bacteroides, and Butyricimonas abundances. q values, after Holm correction for multiple comparisons