Fig. 4
Microbial biological process and cellular component gene ontologies associated with genital inflammatory cytokine profiles. Unsupervised hierarchical clustering of aggregated intensity-based absolute quantification (iBAQ) values for microbial protein a biological process (BP) or b cellular component (CC) gene ontology (GO) relative abundance (n = 113). GO relative abundance was determined by metaproteomics and aggregation of microbial protein iBAQ values assigned to the same GO term. The heatmaps show aggregated microbial GO relative abundance, and the bar graphs show fold changes in aggregated log2-transformed iBAQ values (LOGFC) for each microbial protein with the same a BP or b CC GO in women with high versus low inflammation. Inflammation groups were defined based on hierarchical followed by K-means clustering of women according to the concentrations of nine pro-inflammatory cytokines [interleukin (IL)-1α, IL-1β, IL-6, IL-12p40, IL-12p70, tumor necrosis factor (TNF)-α, TNF-β, TNF-related apoptosis-inducing ligand (TRAIL), interferon (IFN)-γ]. Red bars indicate positive and blue bars indicate negative fold changes in women with high versus low inflammation. False discovery rate-adjusted p values are shown by dots, with red dots indicating low p values. Red arrows indicate cell wall and membrane processes and components. BV bacterial vaginosis, Proinflam cyt pro-inflammatory cytokine