Fungi-Flow discriminates between sporulated and budding fungal forms. a Overnight culture of sporulated forms in RPMI medium. b Acquisition by flow cytometry permits discrimination of two populations according to size (FSC) and granularity (SSC). c Calcofluor White staining of both fungal populations. d Fluorescence-Activated Cell Sorting of two C. albicans and A. fumigatus populations shows a yeast or spores enriched population, which is well differentiated from the budding form population (Light microscopy imaging × 400).