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Table 1 Summary of sampling locations, sponge features classified according to (i) host nutritional mode (presence or absence of photosymbionts); (ii) microbial symbiont strategy (high or low microbial abundance) and sequencing statistics. N50 values for each dataset were calculated based on evaluation of unfiltered contigs

From: Viral ecogenomics across the Porifera

Host species/replicate Sampling site1 Hosts photo-symbionts Microbial abundance Raw reads* (#) Contig N50 Contigs (#) Longest contig (bp) Predicted genes (#) Taxonomically assigned genes (%)
Callyspongia sp. 1 GBR Yes LMA 11.06 899 23,339 58,159 63,281 23.5
Callyspongia sp. 2 GBR Yes LMA 9.36 932 20,309 172,832 56,308 23.6
Callyspongia sp. 3 GBR Yes LMA 8.97 810 21,236 69,654 56,363 24.0
C. foliascens 1 GBR Yes HMA 4.92 675 33,078 24,096 57,288 13.2
C. foliascens 2 GBR Yes HMA 1.78 698 14,225 46,996 25,768 11.0
C. foliascens 3 GBR Yes HMA 15.28 624 45,237 23,964 76,516 13.0
C. schulzei 1 GBR No HMA 7.95 467 9621 80,905 17,001 14.8
C. schulzei 2 GBR No HMA 8.23 571 12,170 81,025 26,002 17.5
C. schulzei 3 GBR No HMA 19.59 660 50,864 80,778 95,389 14.4
C. marshae 1 GBR Yes HMA 1.53 434 4549 9804 7975 16.2
C. marshae 2 GBR Yes HMA 3.75 398 5419 7498 6585 16.6
C. marshae 3 GBR Yes HMA 3.12 405 6751 12,455 10,427 16.1
E. isaaci 1 GBR NA NA 4.20 566 11,349 31,106 21,794 22.5
E. isaaci 2 GBR NA NA 4.50 510 13,898 49,604 28,567 23.3
E. isaaci 3 GBR NA NA 1.26 694 4752 42,237 11,022 21.8
I. basta 1 GBR No LMA 6.58 504 11,600 60,819 24,285 19.3
I. basta 2 GBR No LMA 4.63 495 12,591 39,149 23,850 22.2
I. basta 3 GBR No LMA 4.83 552 14,561 36,340 28,801 20.8
L. herbacea 1 GBR Yes LMA 7.96 457 11,693 43,955 21,907 20.2
L. herbacea 2 GBR Yes LMA 14.88 594 9661 14,898 18,144 19.2
L. herbacea 3 GBR Yes LMA 3.02 504 16,931 43,769 30,283 20.2
P. candelabra 1 GBR NA NA 12.20 693 35,688 49,806 32,910 15.0
P. candelabra2 GBR NA NA 7.74 557 27,010 58,681 48,732 14.4
P. candelabra 3 GBR NA NA 5.87 958 13,373 120,555 29,643 18.2
S. carteri 1 GBR No LMA 6.34 346 4063 10,851 5709 17.6
S. carteri 2 GBR No LMA 4.78 384 7833 16,548 13,143 21.9
A. ochracea 3 Red Sea Yes LMA 3.38 720 7706 26,892 18,040 25.6
A. ochracea1 Red Sea Yes LMA 14.14 717 29,033 61,876 71,543 23.2
A. ochracea 2 Red Sea Yes LMA 2.79 694 7692 20,756 10,981 21.1
C. foliascens 1 Red Sea Yes HMA 2.30 1671 2571 35,948 7096 20.7
C. foliascens 2 Red Sea Yes HMA 1.64 1135 3913 49,410 10,708 19.7
C. foliascens 3 Red Sea Yes HMA 1.82 1280 2787 38,097 7284 21.4
C. cyathophora 1 Red Sea Yes LMA 3.19 601 2757 19,321 8636 25.6
C. cyathophora 2 Red Sea Yes LMA 3.58 618 2613 39,999 5753 24.9
C. cyathophora 3 Red Sea Yes LMA 1.84 614 3977 44,834 7951 21.5
H. erectus 1 Red Sea No HMA 18.37 921 6400 7865 17,674 21.8
H. erectus 2 Red Sea No HMA 6.04 878 9540 47,148 25,550 23.9
Mycale sp. 1 Red Sea NA NA 5.81 949 5396 39,546 13,458 16.1
Mycale sp.2 Red Sea NA NA 5.74 705 9562 54,476 22,104 22.7
Mycale sp. 3 Red Sea NA NA 5.06 682 6123 22,927 13,601 20.2
N. rowi 1 Red Sea NA NA 2.40 668 6058 25,175 13,766 27.5
N. rowi 2 Red Sea NA NA 7.35 922 10,017 52,589 27,503 24.0
X. testudinaria 1 Red Sea Yes HMA 6.13 881 7533 103,486 20,344 16.2
X. testudinaria 2 Red Sea Yes HMA 7.40 729 9639 74,732 23,194 17.1
Sea water—GBR GBR NA NA 21.64 406 11,008 20,625 19,950 22.0
Sea water—RS Red Sea NA NA 9.51 523 10,568 52,589 36,395 35.1
  1. 1GBR sponges were collected from Orpheus Island, Queensland, Australia (18° 35′ 34″ S, 146° 28′ 53″ E) and Red Sea sponges were collected in Al Fahal, Saudi Arabia (22° 13′ 95″ N, 39° 01′ 81″ E)
  2. *Raw read values are presented as million of reads. Taxonomic assignments are based on pairwise sequence similarity to the Viral RefSeq database using MEGAN LCA parameters [30]