Fig. 2

Activity of the different clones quantified in liquid medium toward resorufin-β-GalNAc. Since all the clones isolated at pH 9 were also isolated at pH 8, the activity at pH 8 (at 37 °C in 50 mMTris/HCl) of the 22 different clones identified in this study is presented in this figure. Activity was normalized with cell growth, quantified at DO_600nm before lysing the cells to extract the intracellular GHs. The negative clone Epi (host Epi100 E. coli strain) was used as negative control