Fig. 5

Gut microbiota regulated vitamin B₆ in EphB6-deficient mice. a–d In non-targeted metabolomics analysis, the metabolites in PFC of 8-week-old male WT and KO mice were differently clustered by orthogonal partial least squares discriminant analysis (a). The enriched KEGG pathways associated with differential metabolites (b), the relative abundance of pyridoxamine (PM, c), and pyridoxal 5′-phosphate (PLP, d) were showed. n = 7, 6 mice respectively. e–k The fecal microbiota from 8-week-old WT mice or PBS were gavaged to 8-week-old WT or KO mice for 1 week (e). One week later, the level of PM (f), PLP (g), and pyridoxine (PN, h) in feces of mice were detected. n = 3, 4, 4 mice respectively. The level of PM and PLP in plasma (i, j, n = 5, 5, 6 mice respectively) and level of PLP in PFC (k, n = 4, 5, 6 mice respectively) of mice were also detected. Data shown are mean ± SEM. R (b–d), one-way ANOVA (f–k). *p < 0.05; **p < 0.01; ***p < 0.001. WT, EphB6^+/+ mice; KO, EphB6^−/− mice; FMT fecal microbiota transplantation, PBS phosphate-buffered saline, PFC prefrontal cortex, PM pyridoxamine, PLP pyridoxal 5′-phosphate, PN pyridoxine. Statistical values are presented in Additional file 3: Table S2