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Fig. 4 | Microbiome

Fig. 4

From: A ubiquitous subcuticular bacterial symbiont of a coral predator, the crown-of-thorns starfish, in the Indo-Pacific

Fig. 4

FISH analysis of three serial sections of a COTS disc spine. Each section was hybridized with the EUB338mix (a, purple; a general probe for bacteria), COTSsymb (b, red; a COTS27-specific probe), or Non338 (c, purple; a negative control to detect non-specific binding) probes. The probes were labelled with Cy3 in all panels and coloured with purple in a and c and with red in b. The green signals are tissue-derived autofluorescence. The arrowheads in a and b indicate layer-like signals from the general probe for bacteria (a) and the COTS27- specific probe (b). N.S. and C.U. indicate regions with non-specific binding and the outer cuticle complex, respectively. The scale bars represent 20 μm (a–c)

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Microbiome

ISSN: 2049-2618

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