Fig. 3

The phylogenetic position of the dominant OTU 1 (COTS27) in the phylum Spirochaetes. Maximum likelihood (ML) trees were constructed based on the full-length 16S rRNA gene sequences (a) and the sequences of 43 conserved marker genes identified by CheckM (b). The bootstrap values in a were calculated by resampling 1000 times. The scale bars indicate substitutions per site. *1: The gene with accession No. FQ660021.1 in a was obtained from a polycycle aromatic hydrocarbon (PAH)-contaminated soil sample in a mitigated wetland.