Fig. 2

Functional congruency between the soil and the plant fibre feeder gut metatranscriptomes. a Average cumulative expression of all transcripts annotated to KEGG Ontology categories, for plant fibre- and soil-feeding termite clusters. Transcripts enriched (according to LEfSe analysis [41]) or present exclusively in plant fibre- or soil-feeding termite cluster are marked in green and brown colour, respectively. psmB, proteasome β-subunit; aprX, serine protease; cwlS, peptidoglycan DL-endopeptidase;narG/narZ/nxrA, nitrate reductase/nitrite oxidoreductase α-subunit; nifD and nifK, nitrogenase molybdenum-iron protein chains. b Average cumulative expression of all transcripts with predicted metabolic activity (based on annotation to BRITE database), for plant fibre- or soil-feeding termite clusters. Transcripts annotated to enzyme class EC.3.2.1.x (glycosylases) are marked with black frame around the dot. Transcripts enriched or present exclusively in plant fibre- or soil- feeding termite cluster are marked in green and brown colour, respectively. c Tree based on calculated Bray-Curtis dissimilarity of prokaryotic metatranscriptomic profiles. d Putative taxonomic origin of prokaryotic gene transcripts with KO assignment. Relative abundances of phyla were derived from number of sequencing reads mapped to the de novo re-constructed transcripts. e Major metabolic pathway categories identified in 11 tested prokaryotic microbiomes. For colonies E.neo_1 and S.hey_1, results for the two replicates are presented. f Modules related to carbohydrate metabolism identified in 11 tested prokaryotic microbiomes. For colonies E.neo_1 and S.hey_1, results for the two replicates are presented