Table 4 Antibodies used for FACS
Antigen | Clone | Isotype | Fluorochrome | Supplier |
|---|---|---|---|---|
CD3 | UCHT1 | IgG1,k | PE Cy5 | BD Bioscience |
CD14 | 61D3 | IgG1,k | PE Cy5 | BioRad-Serotec |
CD14 | M5E2 | IgG2a,k | PerCP Cy5.5 | BD Bioscience |
CD14 | MФP9 | IgG2b,k | PECF594 | BD Horizon |
CD16 | 3G8 | IgG1,k | PE Cy5 | BD Bioscience |
CD19 | HIB19 | IgG1,k | PE Cy5 | BD Bioscience |
CD34 | 581 | IgG1,k | PE Cy5 | BD Bioscience |
CD64 | 10.1 | IgG1,k | PerCP Cy5.5 | BD Bioscience |
CD64 | 10.1 | IgG1,k | PE Cy5 | Abcam |
CD123 | 6H6 | IgG1,k | PE Cy7 | eBioscience |
HLA-DR | G46-6 | IgG2a | APC | BD Bioscience |
HLA-DR | L234 | IgG2a | BV421 | Biolegend |
HLA-DR | L234 | IgG2a | BV570 | Biolegend |
CD11c | B-Ly6 | IgG1,k | BV605 | BD Bioscience |
CD40 | LOB7/6 | IgG2a | FITC | AbD Serotec (Bio-Rad) |
CD40 | 5C3 | IgG1 | BV711 | BD Bioscience |
CD45 | H130 | IgG1,k | BUV395 | BD Horizon |
CD80 | L307.4 | IgG1,k | FITC | BD Bioscience |
CD80 | L307.4 | IgG1,k | PE | BD Bioscience |
CD86 | BU63 | IgG1 | FITC | AbD Serotec (Bio-Rad) |
CD86 | 2331(FUN-1) | IgG1,k | AlexaFluor700 | BD Pharmingen |
CD103 (Integrin αE) | Ber-Act8 | IgG1,k | BV421 | Biolegend, BD Bioscience |
IL-6 | MQ2-13A5 | IgG1,k Rat | FITC | Biolegend |
IL-6 | MQ2-13A5 | IgG1,k Rat | PE | eBioscience |
IL-10 | JES-19F1 | IgG1,k Rat | PE | Biolegend |
IL-10 | JES-19F1 | IgG1,k Rat | APC | BD Bioscience/Biolegend |
Integrin β7 | FIB504 | IgG2a, Rat | PE | Biolegend |
Integrin β7 | FIB504 | IgG2a, Rat | FITC | Biolegend |
Integrin β7 | FIB504 | IgG2a, Rat | APC | BD Bioscience |
SIRPα (CD172a/b) | SE5A5 | IgG1,k | PECy7 | Biolegend |