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Table 4 Antibodies used for FACS

From: Bacteroides thetaiotaomicron-derived outer membrane vesicles promote regulatory dendritic cell responses in health but not in inflammatory bowel disease

Antigen Clone Isotype Fluorochrome Supplier
CD3 UCHT1 IgG1,k PE Cy5 BD Bioscience
CD14 61D3 IgG1,k PE Cy5 BioRad-Serotec
CD14 M5E2 IgG2a,k PerCP Cy5.5 BD Bioscience
CD14 MФP9 IgG2b,k PECF594 BD Horizon
CD16 3G8 IgG1,k PE Cy5 BD Bioscience
CD19 HIB19 IgG1,k PE Cy5 BD Bioscience
CD34 581 IgG1,k PE Cy5 BD Bioscience
CD64 10.1 IgG1,k PerCP Cy5.5 BD Bioscience
CD64 10.1 IgG1,k PE Cy5 Abcam
CD123 6H6 IgG1,k PE Cy7 eBioscience
HLA-DR G46-6 IgG2a APC BD Bioscience
HLA-DR L234 IgG2a BV421 Biolegend
HLA-DR L234 IgG2a BV570 Biolegend
CD11c B-Ly6 IgG1,k BV605 BD Bioscience
CD40 LOB7/6 IgG2a FITC AbD Serotec (Bio-Rad)
CD40 5C3 IgG1 BV711 BD Bioscience
CD45 H130 IgG1,k BUV395 BD Horizon
CD80 L307.4 IgG1,k FITC BD Bioscience
CD80 L307.4 IgG1,k PE BD Bioscience
CD86 BU63 IgG1 FITC AbD Serotec (Bio-Rad)
CD86 2331(FUN-1) IgG1,k AlexaFluor700 BD Pharmingen
CD103 (Integrin αE) Ber-Act8 IgG1,k BV421 Biolegend, BD Bioscience
IL-6 MQ2-13A5 IgG1,k Rat FITC Biolegend
IL-6 MQ2-13A5 IgG1,k Rat PE eBioscience
IL-10 JES-19F1 IgG1,k Rat PE Biolegend
IL-10 JES-19F1 IgG1,k Rat APC BD Bioscience/Biolegend
Integrin β7 FIB504 IgG2a, Rat PE Biolegend
Integrin β7 FIB504 IgG2a, Rat FITC Biolegend
Integrin β7 FIB504 IgG2a, Rat APC BD Bioscience
SIRPα (CD172a/b) SE5A5 IgG1,k PECy7 Biolegend
  1. List of all antibodies used to identify and characterise human DC in circulation and in colon for Figs. 1, 2, 3, 4 and 5 and Supplementary Figs 1-4