Fig. 4

Loss of immunoregulatory IL-10 response by circulating DC to Bt OMVs in CD and UC. Whole PBMC were separated from blood of individuals with inactive Crohn’s Disease (CD) or inactive or active ulcerative colitis (UC) and intracellular cytokine analysis of mDC was examined by FACS. a Pooled data from inactive UC (n = 5) and CD (n = 5) patients show proportions of mDC expressing (a) IL-6 in response to Bt and Bt OMVs and compared to HC. b Pooled data from inactive UC (n = 5) show proportions of mDC expressing IL-10 in response to Bt and Bt OMVs and compared to HC. c Pooled data from inactive CD (n = 5) show proportions of mDC expressing IL-10 in response to Bt and Bt OMVs and compared to HC. Statistical significance was determined by Brown-Forsythe and Welch ANOVA for unequal variance with Dunnett’s T3 multiple comparisons test or two-way ANOVA with Holm Sidak’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. d Pooled data from active UC (n = 3) showing proportions of mDC expressing IL-6 (top) or IL-10 (bottom) and comparison to HC. Statistical significance was determined by non-parametric Kruskal-Wallis ANOVA with Dunn’s multiple comparison test; *p < 0.05