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Fig. 2 | Microbiome

Fig. 2

From: Bacteroides thetaiotaomicron-derived outer membrane vesicles promote regulatory dendritic cell responses in health but not in inflammatory bowel disease

Fig. 2

Bt OMVs activate and stimulate both IL-6 and IL-10 expression from healthy circulating DC. PBMC were prepared from healthy individuals. a Following 20 h culture in the presence of Bt or varying concentrations of Bt OMVs, DC were identified within live cells as HLA-DR+ Lineage (CD3/14/16/19/34) cells and further subdivided into CD11c+ myeloid DC or CD123+ pDC. b Pooled data (n = 4) showing proportion of mDC (top panel) and pDC (bottom panel) expressing activation marker CD80. Statistical significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test; *p < 0.05; **p < 0.01. c Intracellular cytokine expression by mDC in response to Bt and OMVs is shown as a proportion of mDC expressing IL-6 (top panel) or IL-10 (bottom panel). Statistical significance was determined by one-way ANOVA with Brown-Forsyth and Welch corrections for unequal variance and with Dunnett’s T3 multiple comparisons tests; *p < 0.05; **p < 0.01; ***p < 0.001. d Total amounts of secreted IL-6 (top panel) and IL-10 (bottom panel) were measured by ELISA in culture supernatants taken at 20 h. Data shown is pooled from n = 3-4 HC. Statistical significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test (IL-10) or non-parametric Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test (IL-6); *p < 0.05, **p < 0.01

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