Fig. 6

Simultaneous depletion of host mitochondrial and chloroplast 16S rRNA genes in phyllosphere samples using Cas-16S-seq. Two gRNAs (mt-gRNA780 and cp-gRNA578) targeting 515F-806R amplicon (V4 region) were used in Cas-16S-seq for three phyllosphere samples (leaf 1–3). The OTU table is rarefied to 50,000 reads (see also Additional file 5: Table S7 and S8). a Fractions of bacterial, mitochondrial, and chloroplast 16S rRNA genes in phyllosphere samples. b Comparison of OTU number with median abundance > 1 in each phyllosphere sample (also see Additional file 5: Table S7). c Comparison of bacterial community structure at family level (Cas9-16S-seq vs regular 16S-seq). The heatmap shows the abundances of bacterial families in each phyllosphere sample. The red box labels the bacterial families which are not observed in regular 16S-seq library